Archives
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br Structural and biochemical features of pol
2021-02-26
Structural and biochemical features of pol X family polymerases The X-family DNA polymerase (polX) is specialized in DNA repair. This family is composed of four different DNA polymerases: pol β, pol λ, pol μ and TdT. Only three members of the polX family possess an N-terminal BRCT (BRCA1 carboxy-
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Diosmetin In this review focusing on biocatalyst formate deh
2021-02-26
In this review, focusing on biocatalyst “formate dehydrogenase FDH” catalyzing both of the formic Diosmetin oxidation to CO2 and the CO2 reduction to formic acid, representative examples of properties, types, structure of active-site of FDH and, reaction mechanism of formic acid oxidation and CO2 re
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br Preparation of Recombinant Proteins br Activity Assays Th
2021-02-26
Preparation of Recombinant Proteins Activity Assays The following solvents are used in the procedures described in this section: Data Analysis Aryl radical rearrangements typically result in a large number of products being generated by Bmp7. Approaches combining mass spectrometry and NMR
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A more refined picture of cholinesterase
2021-02-26
A more refined picture of cholinesterase activity patterns may be obtained by measuring ChE activity of the same sample before and after addition of certain cholinesterase inhibitors, with differential specificity for different components of total ChE activity, such as BW284c51 (BW1,5-bis-(4-allyldi
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In the context of our ongoing
2021-02-26
In the context of our ongoing studies to define the phytochemical and biological properties of oxyprenylated secondary metabolites of plant and fungal origin, we wish to report herein the investigation on the interaction of some natural and semisynthetic O-alkylcoumarins with promising ChE inhibitor
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rad001 Similarly in the EAE model Chalmin et al previously d
2021-02-26
Similarly in the EAE model, Chalmin et al. previously demonstrated that EBI2 and CH25H might be responsible for the efficient egress of differentiated Th17 cells from the draining lymph nodes [31]. As discussed above, in active EAE we did not find differences in EAE development and only the transfer
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br Results br Discussion It has been thought that
2021-02-26
Results Discussion It has been thought that commitment to the plasma cell fate begins while monensin are still in the GC (Suan et al., 2017, Victora and Nussenzweig, 2012), but the main obstacle to test this model and, if correct, to clarify how the plasma cell-prone GC cells develop has been
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br Results br Discussion The ubiquitin
2021-02-26
Results Discussion The ubiquitin system has in recent years become an exciting area for drug discovery (Cohen and Tcherpakov, 2010), as multiple enzymatic steps within the ubiquitylation process are druggable. The potential of targeting the ubiquitin-proteasome pathway was first demonstrated i
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br Application of D receptor PET in psychiatric
2021-02-26
Application of D1-receptor PET in psychiatric disorders Discussion Compared to other markers of the DA system, only a limited number of studies have investigated D1-R in psychiatric disorders. In schizophrenia, which is the most studied condition with a total of 83 identified unique patients,
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Why then is leading strand DNA synthesis reduced relative
2021-02-25
Why then is leading-strand DNA synthesis reduced relative to lagging-strand synthesis in rad53-1 mutant antioxidant under replication stress? To gain insight into this question, we first determined whether the firing of late origins in rad53-1 mutant cells contributes to compromised leading-strand
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MTX and MTXPGs block the activity of the key enzyme
2021-02-25
MTX and MTXPGs block the activity of the key enzyme DHFR (Fig. 1), which converts folates to their active forms – dihydrofolate (DHF) and tetrahydrofolate (THF). MTXPGs also potently inhibit thymidylate synthase (TS). Furthermore, during dTMP synthesis, TS utilizes the cofactor 5,10-methylene THF, w
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The in vivo IC M in the adipose tissue
2021-02-25
The in vivo IC (0.0003μM) in the adipose tissue assay is in very good agreement with the in vitro IC (0.0005μM), and it is larger than the one measured in the OLTT assay (0.00005μM) for this compound. These differences in in vivo IC between OLTT and TAG synthesis are expected as we used free SEA0400
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endothelin receptor antagonist As part of a program to
2021-02-25
As part of a program to explore the key binding interactions between the core of 1 and DGAT-1 a systematic analysis of the aminopyrimidine subunit was initiated. A potential approach to improving passive permeability within this series would be to increase lipophilicity via incorporation of substitu
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br Results br Discussion Prostate cancer dissemination
2021-02-25
Results Discussion Prostate cancer dissemination and skeletal metastases represent major therapeutic challenges. Once the skeletal metastasis occurs, the incurable patient could just be symptomatically treated to alleviate the pain. Therefore it is of great necessity to disclose the molecular
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Actinomycin D In a study on the time
2021-02-25
In a study on the time course of a mechanism-based inactivation of CYP3A by HIV protease inhibitors, Katzenmaier et al. [37] reported that the suppression of CYP3A activity continued for threedays after the discontinuation of ritonavir. In the present study, the CYP3A activity on day 28 after a seve